The Feasibility of Using Human Primary Chondrocytes Derived from Osteoarthritic Patients Overexpressed with SOX9 Seeded on PLGA-Fibrin Hybrid Scaffolds for Cartilage Engineering

This study aimed to find an optimal formulation form 3D hyaline-like cartilage substitutes using the tissue engineering triads. The primary cells taken from osteoarthritic patients were overexpressed with transcriptional factor SRY (Sex Determining Region Y)-Box 9 (SOX9) Lipofectamine 2000™ through a non-viral transfection method. transfected and non-transfected seeded on poly(lactic-co-glycolic acid) (PLGA) based scaffolds without fibrin. arrangement resulted in four experimental groups. ‘cells-scaffolds’ constructs cultured for three weeks implanted ectopically nude mice weeks. evaluations include macroscopic microscopic study, gene expression analyses, sulfated glycosaminoglycan (sGAG) assay, focusing properties. A biomechanical evaluation was performed only post-implanted constructs. All vitro, two- four-week exhibited firm smooth appearance. In vitro showed sparse distribution minimal cartilaginous formation. However, high density, lacunae-encapsulated chondrocytes embedded within basophilic ground substances observed all It is supported by positive-brownish precipitation immunolocalisation against collagen type II. Besides, molecular analysis that COL2A1 other markers also expressed. Increased sGAG content compressive strain could be vivo. Although quantitatively, no significant statistical differences found between groups, qualitative results indicated SOX9-overexpressed cells, PLGA, fibrin combination guides formation better than Hence, may studied big animal model develop its potential future clinical application.